Solid-phase derivatization of thiols : Application to high-performance liquid chromatography
โ Scribed by Marie-Claude Millot; Bernard Sebille; Jean-Pierre Mahieu
- Book ID
- 108336585
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 759 KB
- Volume
- 354
- Category
- Article
- ISSN
- 1873-3778
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๐ SIMILAR VOLUMES
## An automated pre-column derivatization system is described. Problems arising from the coupling of this AutoAnalyzer-type system with high-performance liquid chromatography are discussed in detail. The system has been applied to ionpair partition chromatography. The ion-pairs of a mixture of so
Three N-substituted maleimides were tested as derivatizing reagents; N-(4anilinophenyl) maleimide (APM) was the most favorable in terms of sensitivity and reactivity. N-Acetyl-L-cysteine, glutathione, L-cysteine and D-penicillamine were readily converted into the adducts with APM. Picogram levels of