Sensitive derivatization reagents for thiol compounds in high-performance liquid chromatography with electrochemical detection

✍ Scribed by Kazutake Shimada; Makoto Tanaka; Toshio Nambara

• Publisher: Elsevier Science
• Year: 1983
• Tongue: English
• Weight: 385 KB
• Volume: 147
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(83)80107-x

✦ Synopsis

Three N-substituted maleimides were tested as derivatizing reagents; N-(4anilinophenyl) maleimide (APM) was the most favorable in terms of sensitivity and reactivity. N-Acetyl-L-cysteine, glutathione, L-cysteine and D-penicillamine were readily converted into the adducts with APM. Picogram levels of these thiol compounds were separated and quantified.