Automation of pre-column derivatization in high-performance liquid chromatography : Application to ion-pair partition chromatography

✍ Scribed by J.C. Gfeller; J.M. Huen; J.P. Thevenin

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 452 KB
• Volume: 166
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)92256-8

✦ Synopsis

An automated

pre-column derivatization system is described. Problems arising from the coupling of this AutoAnalyzer-type system with high-performance liquid chromatography are discussed in detail. The system has been applied to ionpair partition chromatography.

The ion-pairs of a mixture of some alkaloids (apoatropine, hyoscyamine, scopolamine and ergotamine) were formed with picric acid in an aqueous phase, extracted into chloroform and injected on-line on to a silica gel column for separation within 15 minutes. This study demonstrates the possibility of obtaining an automated pre-column derivatization system with a good relative standard deviation of ( 3"/d, per injection (n = 6). The linearity of the system for hyoscyamine and ergotamine is significant in the concentration ranges of S&ZOO ng and 240-720 n_g per injection, respectively. The experience gained is of general validity and could generate more studies in this area.