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Sodium iodide density gradients for the preparative buoyant density separations of dna mixtures

✍ Scribed by Ragini Anet; David R. Strayer


Book ID
118851473
Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
318 KB
Volume
37
Category
Article
ISSN
0006-291X

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A rapid and easy method for the isolation of plasmid DNA, both in analytical and preparative scale, is described. Using dye-buoyant density-gradient centrifugation in a vertical rotor, separation of covalentlv closed. sunercoiled plasmid DNA from relaxed circular and linear DNA is completed within 1