An efficient method for the isolation of RNA from cartilage is described. The difficulties in obtaining RNA from cartilage, a tissue of low cell density and high proteoglycan content, were overcome by making several modifications to the guanidine thiocyanate/cesium chloride method of RNA extraction.
Preparative density gradient centrifugation of RNA and DNA in cesium sulfateurea mixture
โ Scribed by Sohan P. Modak; Maria-Tereza Imaizumi; Monique Chappuis; Klaus Scherrer
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 412 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0301-4851
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A procedure for the simultaneous banding of cellular DNA, RNA, and protein by centrifugation in cesium trifluoracetate (CsTFA) gradients is described. Starting with homogenates of Day 11 rat embryos, this procedure was used to separate total DNA, RNA, and protein. Under the conditions used DNA bande
## Abstract A density gradient apparatus was used to examine the separation of different physical forms and sizes of DNA. A gradient of sucrose was used to stabilize thermal convection during electrophoresis in the column (2.2 cm in diameter). Linear polymers were added to the density gradient and