Sodium dodecyl sulfate-capillary gel electrophoresis of proteins using non-cross-linked polyacrylamide
β Scribed by Dan Wu; Fred E. Regnier
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 605 KB
- Volume
- 608
- Category
- Article
- ISSN
- 1873-3778
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Capillary sodium dodecyl sulfate-gel electrophoresis, a one-dimensional version of the well-established planar analytical method of SDS-polyacrylamide gel electrophoresis, has proven a powerful new microanalytical method for the separation of protein molecules according to their size. In this paper
Gel electrophoresis has been used extensively as an analytical technique to check the purity and to determine the molecular weight of proteins. Improved levels of detection of proteins by silver staining of the gels have made the technique more sensitive in detecting heterogeneity. We report here so
The performance of capillary gel-sodium dodecyl sulfate (SDS) electrophoresis for molecular-mass-dependent separation of proteins has been examined employing cross-linked polyacrylamide gels and untreated fused-silica capillaries of short length. An apparatus for capillary electrophoresis has been c