✦ LIBER ✦

Multiple Bands on the Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Gels of Proteins Due to Intermolecular Disulfide Cross-Linking

✍ Scribed by T.K.S. Kumar; K. Gopalakrishna; V.V.H. Prasad; M.W. Pandit

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 289 KB
Volume: 213
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1413

No coin nor oath required. For personal study only.

✦ Synopsis

Gel electrophoresis has been used extensively as an analytical technique to check the purity and to determine the molecular weight of proteins. Improved levels of detection of proteins by silver staining of the gels have made the technique more sensitive in detecting heterogeneity. We report here some interesting observations about the anomalous behavior of some proteins in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). This report is concerned with the appearance of multiple bands, after silver staining, on the SDS-PAGE gels of proteins which are shown by HPLC to be homogeneous. We also show the reasons for the appearance of bands that originate from 2-mercaptoethanol itself. 1983 Academic Press, Inc.

📜 SIMILAR VOLUMES

Urea Reduces the Aggregation of Membrane

Urea Reduces the Aggregation of Membrane Proteins on Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis

✍ Stéphanie Soulié; Jesper Vuust Møller; Pierre Falson; Marc le Maire 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 70 KB

Sodium dodecyl sulfate electrophoresis o

Sodium dodecyl sulfate electrophoresis of high molecular weight proteins in N, N'-diallyltartardiamide-cross-linked polyacrylamide slab gels

✍ A. Ziegler; H. Hengartner 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 85 KB 👁 2 views

Modification of the Laemmli Sodium Dodec

Modification of the Laemmli Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Procedure to Eliminate Artifacts on Reducing and Nonreducing Gels

✍ Susan Cannon-Carlson; John Tang 📂 Article 📅 1997 🏛 Elsevier Science 🌐 English ⚖ 107 KB

Alteration in the electrophoretic mobili

Alteration in the electrophoretic mobility of OmpC due to variations in the ammonium persulfate concentration in sodium dodecyl sulfate-polyacrylamide gel electrophoresis

✍ Sergio R. Lobos; Dr. Guido C. Mora 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 369 KB

Analysis of polymeric proteins by two-di

Analysis of polymeric proteins by two-dimensional polyacrylamide gel electrophoresis on one slab in the presence and absence of sodium dodecyl sulfate

✍ Dr. Hans Bloemendal; Kees Jansen 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 211 KB 👁 2 views

Requirements for the application of prot

Requirements for the application of protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis and randomly amplified polymorphic DNA analyses to product speciation

✍ Iciar Martinez; Tone Jakobsen Friis; Marit Seppola 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 241 KB 👁 2 views

## Requirements for the application of protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis and randomly amplified polymorphic DNA analyses to product speciation Raw, cooked, fried, smoked and gravad (brine-cured) products were analyzed by Sodium dodecyl sulfate-polyacrylamide gel ele