Modification of the Laemmli Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis Procedure to Eliminate Artifacts on Reducing and Nonreducing Gels
✍ Scribed by Susan Cannon-Carlson; John Tang
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 107 KB
- Volume
- 246
- Category
- Article
- ISSN
- 0003-2697
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📜 SIMILAR VOLUMES
Gel electrophoresis has been used extensively as an analytical technique to check the purity and to determine the molecular weight of proteins. Improved levels of detection of proteins by silver staining of the gels have made the technique more sensitive in detecting heterogeneity. We report here so
method of de Vendittis et al. (7). ## Polyacrylamide Gel Electrophoresis and a Figure 1A (lanes 1 -5) shows the effect of increas- ## Method for Eliminating This Effect ing concentrations of PEG from 0 to 2.0% on the mobility of the standard proteins. The mobilities of the three largest protei