✦ LIBER ✦
An Effect of Polyethylene Glycol 8000 on Protein Mobility in Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis and a Method for Eliminating This Effect
✍ Scribed by Obed W. Odom; Wieslaw Kudlicki; Gisela Kramer; Boyd Hardesty
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 193 KB
- Volume
- 245
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
method of de Vendittis et al. (7).
Polyacrylamide Gel Electrophoresis and a
Figure 1A (lanes 1 -5) shows the effect of increas-
Method for Eliminating This Effect
ing concentrations of PEG from 0 to 2.0% on the mobility of the standard proteins. The mobilities of the three largest proteins increase with PEG, while Obed W. Odom, Wieslaw Kudlicki, Gisela Kramer, those of the two smallest proteins decrease. The perand Boyd Hardesty 1 cent change in apparent molecular weight is greatest