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SmaRT-PCR: a novel application of competitive PCR for mRNA quantitation

โœ Scribed by Marten Szibor; Henning Morawietz

Book ID
114393118
Publisher
Elsevier
Year
2001
Weight
192 KB
Volume
6
Category
Article
ISSN
1366-2120

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A novel approach of compelitive PCR for quantifying mRNAs is described where competitors are constructed by deleting an internal sequence from target reverse transcribed mRNAs. The two sets of product generated (control and target) are easily discriminated by size and quantified for the sequence of