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PCR Protocols || COMPETITIVE PCR FOR QUANTITATION OF mRNA

โœ Scribed by Gilliland, Gary


Book ID
118241654
Publisher
Elsevier
Year
1990
Weight
84 KB
Category
Article
ISBN
0123721806

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A novel approach of compelitive PCR for quantifying mRNAs is described where competitors are constructed by deleting an internal sequence from target reverse transcribed mRNAs. The two sets of product generated (control and target) are easily discriminated by size and quantified for the sequence of