Rapid construction of competitive templates for quantitative PCR of reverse-transcribed mRNAs.

✍ Scribed by Luca Giacco; Luisa Doneda; Paola Grammatico; Lidia Larizza

• Book ID: 104648248
• Publisher: Springer-Verlag
• Year: 1995
• Tongue: English
• Weight: 503 KB
• Volume: 9
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00158540

✦ Synopsis

A novel approach of compelitive PCR for quantifying mRNAs is described where competitors are constructed by deleting an internal sequence from target reverse transcribed mRNAs. The two sets of product generated (control and target) are easily discriminated by size and quantified for the sequence of interest and a housekeeping gene, p-actin ,used as internal mllNA control.