Small-angle X-ray scattering of human serum high-density lipoproteins

✍ Scribed by Shipley, G. G. ;Atkinson, D. ;Scanu, A. M.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1972
• Tongue: English
• Weight: 332 KB
• Volume: 1
• Category: Article
• ISSN: 0091-7419
• DOI: 10.1002/jss.400010203

✦ Synopsis

Smal I -a n g l e x -r a y s c a t t e r i n g (SAXRS) s t u d i e s o f t h e human serum h i g h -d e n s i t y l i p o p r o t e i n HDL2 i n d i c a t e a symmetrical p a r t i c l e w i t h a r a d i u s o f g y r a t i o n Rg = 46 A. t i e s o f s u b s i d i a r y maxima i n t h e s c a t t e r i n g curves a r e n o t cons i s t e n t w i t h those o f a u n i f o r m l y e l e c t r o n dense sphere. Scatt e r i n g curves c a l c u l a t e d f o r spheres w i t h a step-model r a d i a l e l e c t r o n d e n s i t y d i s t r i b u t i o n , show good agreement w i t h t h e experimental s c a t t e r i n g c u r v e f o r HDL2 o n l y f o r s p e c i f i c v a l u e s o f t h e s t e p f u n c t i o n used. The dimensions o b t a i n e d f o r t h e e l e c t r o n -d e f i c i e n t c o r e and e l e c t r o n -r i c h s h e l l model a r e q u a n t it a t i v e l y c o n s i s t e n t w i t h a p r e d o m i n a n t l y s u r f a c e l o c a t i o n f o r t h e HDL2 p r o t e i n and p h o s p h o l i p i d head groups, t h e more hydrocarbon species b e i n g l o c a t e d i n t h e i n t e r i o r of t h e p a r t i c l e .