Small-Angle X-Ray Scattering on Solutions of Carboxymethylcellulose and Bovine Serum Albumin

✍ Scribed by Sabrina M. Pancera; Rosangela Itri; Denise F. S. Petri

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 137 KB
• Volume: 5
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.200400206

✦ Synopsis

Abstract

Summary: The existence of attractive interaction between CMC and BSA was evidenced in solution at pH higher than the protein isoelectric point by means of SAXS. Mixtures of BSA at 10 × 10^−3^ g · mL^−1^ and CMC at the concentration range of 1 × 10^−3^ g · mL^−1^ to 10 × 10^−3^ g · mL^−1^ were investigated. Upturns in the very low q range revealed the presence of aggregates when the CMC concentration was higher than 2 × 10^−3^ g · mL^−1^. The comparison between the calculated form factor with the experimental curves at intermediate and long q values indicated that the BSA molecules keep their native form in mixtures with CMC concentrations lower than 5 × 10^−3^ g · mL^−1^. Therefore, for CMC concentrations higher than 2 × 10^−3^ g · mL^−1^ the mixtures start to present aggregates and free BSA molecules coexisting in solution.

SAXS evidenced the complex formation between BSA and carboxymethylcellulose (CMC) at a stoichiometric ratio. Excess of CMC led to aggregation.

magnified imageSAXS evidenced the complex formation between BSA and carboxymethylcellulose (CMC) at a stoichiometric ratio. Excess of CMC led to aggregation.