Size Exclusion Chromatography

✍ Scribed by Prof. Sadao Mori, Dr. Howard G. Barth (auth.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 1999
• Tongue: English
• Leaves: 235
• Series: Springer Laboratory
• Edition: 1
• Category: Library

✦ Synopsis

Although size exclusion chromatography (SEC) is perhaps the most popular and widely used technique for determining the molecular weight distribution of polymeric materials, there have been very few texts written on this topic. During the past decade, SEC has experienced a considerable amount of growth in regard to column and detector technology and new applications. With these advances, SEC can now be used for determining absolute molecular weight, polymer chain conformation and size, and branching, as well as polymer solution properties. This book introduces the reader to the fundamentals of SEC with emphasis on practical aspects of the technique, such as column and mobile selection, calibration, new detector capabilities and guidelines for performing SEC on most types of polymers, especially those of industrial importance. This book is intended for either those new to the field of SEC, or for those research workers who require a more comprehensive background.

✦ Table of Contents

Front Matter....Pages I-XIV
Introduction....Pages 1-9
Fundamental Concepts....Pages 11-29
Instrumentation....Pages 31-44
Columns....Pages 45-53
SEC Method Development....Pages 55-76
Molecular Weight Averages and Distribution....Pages 77-94
Approaches to Molecular Weight Calibration....Pages 95-113
Molecular-Weight-Sensitive Detectors....Pages 115-129
Synthetic Polymers....Pages 131-153
High-Temperature Size Exclusion Chromatography....Pages 155-165
Aqueous Size Exclusion Chromatography....Pages 167-183
Special Applications....Pages 185-198
Back Matter....Pages 199-234

✦ Subjects

Polymer Sciences; Analytical Chemistry; Biotechnology; Biochemistry, general