Aqueous Size-Exclusion Chromatography

✍ Scribed by P.L. Dubin (Eds.)

Publisher: Elsevier; Distributors for the U.S. and Canada, Elsevier Science Pub. Co
Year: 1988
Tongue: English
Leaves: 473
Series: Journal of chromatography library 40
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses. These developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography.

✦ Table of Contents

Content:
Edited by
Page iii

Copyright page
Page iv

Preface
Pages xiii-xvi
Paul Dubin

List of Contributors
Pages xvii-xviii

Chapter 1 Size Exclusion Parameters Original Research Article
Pages 3-22
Michael E. Himmel, Phil G. Squire

Chapter 2 Partitioning: Hydrophobic Interactions Original Research Article
Pages 23-54
Masanobu Janado

Chapter 3 Electrostatic Effects Original Research Article
Pages 55-75
Paul L. Dubin

Chapter 4 Exclusion Chromatography of Inorganic Compounds Original Research Article
Pages 77-115
Masami Shibukawa, Naoichi Ohta

Chapter 5 Pore Size Distributions Original Research Article
Pages 119-155
Lars Hagel

Chapter 6 Structural Analysis of Porous Materials By Measurement of Size Exclusion Original Research Article
Pages 157-170
Shigenori Kuga

Chapter 7 Column Efficiency Original Research Article
Pages 171-190
Sadao Mori

Chapter 8 Native and Bonded Silicas in Aqueous Sec Original Research Article
Pages 193-234
K.K. Unger, J.N. Kinkel

Chapter 9 Rigid Polymer Gels For Sec And Their Application To Biopolymers Original Research Article
Pages 235-266
Keisuke Makino, Hiroyuki Hatano

Chapter 10 Biopolymers. I. Protein Chromatography in Denaturing And Nondenaturing Solvents Original Research Article
Pages 269-296
Ronald C. Montelaro

Chapter 11 Biopolymers. Ii. Serum Lipoproteins Original Research Article
Pages 297-326
Mitsuyo Okazaki, Ichiro Hara

Chapter 12 Application of Sec/Lalls To Biopolymer Assemblies Original Research Article
Pages 327-342
Katsutoshi Konishi

Chapter 13 Measurement of Equilibrium Constants by Exclusion Methods Original Research Article
Pages 345-373
T.K. Korpela, J.‐P. Himanen

Chapter 14 Frontal Boundary Analysis In Size Exclusion Chromatography of Self-Associating Proteins Original Research Article
Pages 375-397
Gerald W. Becker

Chapter 15 Exclusion Chromatography of Micelles Original Research Article
Pages 399-439
K.S. Birdi

Subject Index
Pages 441-449

Journal of Chromatography Library
Pages 451-453

✦ Subjects

Химия и химическая промышленность;Аналитическая химия;Физические методы анализа;Хроматография и масс-спектрометрия;

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