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Site-specific cloning of chromosomal genes of Escherichia coli utilizing gene fusions and plasmid rescue

✍ Scribed by Saarilahti, Hannu T.; Palva, E.Tapio

Book ID
122445293
Publisher
Elsevier Science
Year
1985
Weight
309 KB
Volume
2
Category
Article
ISSN
0735-0651

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recA Gene dependence of replication of t
recA Gene dependence of replication of the Escherichia coli chromosome initiated by plasmid pUC13 integrated at predetermined sites
✍ Mao, Yu-Min ;Shi, Qin ;Li, Qing-Ge ;Sheng, Zu-Jia πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 827 KB

Plasmid pUC13 was used to clone DNA fragments of known sites from the chromosome of Escherichia coli. Each chimeric plasmid was introduced individually into the same dnaA46 mutant strain LC381 and suppressive integration (Sin) strains were selected. By means of cotransduction the null mutation recA5