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Cloning and Expression of a Urate Oxidase and Creatinine Hydrolase Fusion Gene in Escherichia coli

✍ Scribed by Cheng, Xin; Liu, Fang; Zhang, Yanxin; Jiang, Yunsheng

Book ID
120039704
Publisher
Informa plc
Year
2013
Tongue
English
Weight
268 KB
Volume
35
Category
Article
ISSN
0886-022X

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A partial EcoRI fragment of Bacillus coagulans DNA cloned in an Escherichia coli K12 bacteriophage lambda host-vector system was shown to direct the synthesis of a thermostable alpha-amylase whose activity could be detected in situ on petri plates using the iodine staining method. A 3.31 kb EcoRI fr