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Cloning and expression of mycobacterial glutamine synthetase gene in Escherichia coli

✍ Scribed by Jitendra Singh; Mohan Chandra Joshi; Rakesh Bhatnagar


Book ID
116288207
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
250 KB
Volume
317
Category
Article
ISSN
0006-291X

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The entire structural gene for tyrocidine synthetase 1 from Bacillus brevis ATCC 8185 has been cloned and expressed in Escherichia coli. Transformed E. coli cells were screened for their ability to produce tyrocidine synthetase 1 by in situ immunoassay using antibodies against gramicidin S synthetas