𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Introduction, rescue and expression of plasmid genes in mammalian cells and Escherichia coli

✍ Scribed by Richard A. Zakour; Roel M. Schaaper; Barry W. Glickman

Book ID
113192989
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
915 KB
Volume
163
Category
Article
ISSN
0027-5107

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

Rescue of transfected genes from mammali
Rescue of transfected genes from mammalian cells by functional selection in Escherichia coli
✍ Strauss, Michael ;Klessling, Udo ;Kachler, Ruth πŸ“‚ Article πŸ“… 1985 πŸ› Springer 🌐 English βš– 542 KB

We have established procedures for reisolating a transfected gene from mammalian cells by selection in Escherichia coli for the function of the gene product using the Herpes simplex virus thymidine kinase gene as a model. Rescue of the gene is accomplished by three different methods. The tk gene is

Construction of hybrid plasmids containi
Construction of hybrid plasmids containing the lysA gene of Escherichia coli: Studies of expression in Escherichia coli and Saccharomyces cerevisiae
✍ Chenais, JoΓ«l ;Richaud, Catherine ;Ronceray, Jeanine ;Cherest, HΓ©lΓ¨ne ;Surdin-Ke πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 639 KB

The lysA gene of Escherichia coli has been cloned from a lambda transducing phage on various plasmids, present in different copy numbers in bacterial cells. Synthesis of the product of this gene, diaminopimelate (DAP)-decarboxylase, and its regulation have been studied. Expression does not follow a