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Site-directed mutagenesis by double polymerase chain reaction

โœ Scribed by Sailen Barik


Book ID
112902378
Publisher
Humana Press Inc
Year
1995
Tongue
English
Weight
593 KB
Volume
3
Category
Article
ISSN
1073-6085

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Combinatorial alanine scanning mutagenesis is a powerful tool for the exploration of protein structurefunction relationships. Unfortunately, combinatorial alanine replacement of multiple residues using standard site-directed mutagenesis is restricted to a subset of amino acids. To circumvent this li