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Polymerase Chain Reaction-Based Site-Directed Mutagenesis Using Magnetic Beads

โœ Scribed by Jacques Fieschi; Patricia Niccoli; Christophe Camilla; Patrick Chames; Martine Chartier; Daniel Baty

Book ID
102559731
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
97 KB
Volume
234
Category
Article
ISSN
0003-2697

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Combinatorial alanine scanning mutagenesis is a powerful tool for the exploration of protein structurefunction relationships. Unfortunately, combinatorial alanine replacement of multiple residues using standard site-directed mutagenesis is restricted to a subset of amino acids. To circumvent this li