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Generation of Site-Directed Mutagenesis by Extralong, High-Fidelity Polymerase Chain Reaction

โœ Scribed by Nianli Sang; Gianluigi Condorelli; Antonio De Luca; Timothy K. MacLachlan; Antonio Giordano


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
87 KB
Volume
233
Category
Article
ISSN
0003-2697

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Alpha-1 -antitrypsin (A1 AT) deficiency is an autosomal hereditary disorder with a reduction in serum A1 AT levels. In a large family, we used a polymerase chain reaction (PCR)- mediated, site-directed mutagenesis assay to detect the two most common AlAT deficient variants, Z and S. By coamplificati