Single-cell Sequencing and Methylation: Methods and Clinical Applications

✍ Scribed by Buwei Yu, Jiaqiang Zhang, Yiming Zeng, Li Li, Xiangdong Wang

• Publisher: Springer Singapore;Springer
• Year: 2020
• Tongue: English
• Leaves: 251
• Series: Advances in Experimental Medicine and Biology 1255
• Edition: 1st ed.
• Category: Library

✦ Synopsis

With the rapid development of biotechnologies, single-cell sequencing has become an important tool for understanding the molecular mechanisms of diseases, defining cellular heterogeneities and characteristics, and identifying intercellular communications and single-cell-based biomarkers. Providing a clear overview of the clinical applications, the book presents state-of-the-art information on immune cell function, cancer progression, infection, and inflammation gained from single-cell DNA or RNA sequencing. Furthermore, it explores the role of target gene methylation in the pathogenesis of diseases, with a focus on respiratory cancer, infection and chronic diseases. As such it is a valuable resource for clinical researchers and physicians, allowing them to refresh their knowledge and improve early diagnosis and therapy for patients.

✦ Table of Contents

Front Matter ....Pages i-viii
Understanding Diseases from Single-Cell Sequencing and Methylation (Buwei Yu, Li Li, Jiaqiang Zhang, Xiangdong Wang, Yiming Zeng)....Pages 1-6
Methods for Single-Cell Isolation and Preparation (Daniel Pensold, Geraldine Zimmer-Bensch)....Pages 7-27
Single-Cell Sequencing of T cell Receptors: A Perspective on the Technological Development and Translational Application (Shivai Gupta, Richard Witas, Alexandria Voigt, Touyana Semenova, Cuong Q. Nguyen)....Pages 29-50
DNA Methylation in Pulmonary Fibrosis (Shuang Zhou, Xiangdong Wang, Hongzhi Gao, Yiming Zeng)....Pages 51-62
Methylation of Inflammatory Cells in Lung Diseases (Yifei Liu, Hongzhi Gao, Xiangdong Wang, Yiming Zeng)....Pages 63-72
Research Advances on DNA Methylation in Idiopathic Pulmonary Fibrosis (Qian-Kun Luo, Hui Zhang, Li Li)....Pages 73-81
DNA Methylation in Chronic Obstructive Pulmonary Disease (Xiaoyang Chen, Furong Yan, Xiaoping Lin, Liyong Shi, Xiangdong Wang, Yiming Zeng)....Pages 83-98
The Role of RASSF1 Methylation in Lung Carcinoma (Tingting Zhang, Yanjun Li, Hui Zhang, Xiangdong Wang, Xiaozhuan Liu, Li Li)....Pages 99-108
Single Cell Sequencing: A New Dimension in Cancer Diagnosis and Treatment (Fengying Wu, Jue Fan, Jingwen Fang, Priya S. Dalvi, Margarete Odenthal, Nan Fang)....Pages 109-121
The Role of Methylation in the CpG Island of the ARHI Promoter Region in Cancers (Xiaozhuan Liu, Tingting Zhang, Yanjun Li, Yuwei Zhang, Hui Zhang, Xiangdong Wang et al.)....Pages 123-132
Clinical Significance of P16 Gene Methylation in Lung Cancer (Yanjun Li, Tingting Zhang, Hui Zhang, Xiangdong Wang, Xiaozhuan Liu, Qihong Huang et al.)....Pages 133-142
Application of Single-Cell RNA Sequencing in Pancreatic Cancer and the Endocrine Pancreas (Qiankun Luo, Qiang Fu, Xu Zhang, Hongwei Zhang, Tao Qin)....Pages 143-152
Single-Cell Sequencing in Genitourinary Malignancies (Neal Murphy, Pratik Shah, Andrew Shih, Houman Khalili, Anthony Liew, Xinhua Zhu et al.)....Pages 153-164
PI3K Isoform-Selective Inhibitors in Cancer (Leslie Duncan, Chloe Shay, Yong Teng)....Pages 165-173
Single Cell Sequencing in Cancer Diagnostics (Frederik Otzen Bagger, Victoria Probst)....Pages 175-193
Single Cell RNA Sequencing in Human Disease: Renal, Pancreatic, and Viral Diseases (Sayra Garcia, Evan Der, Chaim Putterman)....Pages 195-202
Single-Cell Sequencing in Human Genital Infections (Reema Singh)....Pages 203-220
Emerging Strategies for Therapeutic Antibody Discovery from Human B Cells (Saravanan Rajan, William F. Dall’Acqua)....Pages 221-230
Values of Single-Cell RNA Sequencing in Development of Cerebral Cortex (Enqiang Chang, Xiaoguo Ruan, Ruilou Zhu, Yangyang Wang, Jiaqiang Zhang)....Pages 231-247

✦ Subjects

Biomedicine; Molecular Medicine; Bioinformatics; Cancer Research