This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reacti
Single Cell Diagnostics: Methods and Protocols
β Scribed by David K. Gardner PhD (auth.), Alan Thornhill PhD, HCLD (eds.)
- Publisher
- Humana Press
- Year
- 2007
- Tongue
- English
- Leaves
- 184
- Series
- Methods in Molecular Medicineβ’ 132
- Edition
- 1
- Category
- Library
No coin nor oath required. For personal study only.
β¦ Synopsis
The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.
β¦ Table of Contents
Front Matter....Pages i-xi
Back Matter....Pages 1-9
....Pages 11-18
β¦ Subjects
Human Genetics; Molecular Medicine; Cell Biology
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