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Single Cell Diagnostics: Methods and Protocols

✍ Scribed by David K. Gardner PhD (auth.), Alan Thornhill PhD, HCLD (eds.)


Publisher
Humana Press
Year
2007
Tongue
English
Leaves
184
Series
Methods in Molecular Medicineβ„’ 132
Edition
1
Category
Library

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✦ Synopsis


The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence in situ hybridization and polymerase chain reaction (PCR) As the reader moves through the book, the scope and complexity of each technique gradually increases as real-time quantitative PCR, isothermal whole genome amplification, comparative genomic hybridization, real-time gene expression analysis and the production of RNA and cDNA libraries are covered. The book closes with the application of customized microarrays to the study of single cells.

✦ Table of Contents


Front Matter....Pages i-xi
Back Matter....Pages 1-9
....Pages 11-18

✦ Subjects


Human Genetics; Molecular Medicine; Cell Biology


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