Single Cell Protein Analysis: Methods and Protocols
β Scribed by Anup K. Singh, Aarthi Chandrasekaran
- Publisher
- Humana Press
- Year
- 2015
- Tongue
- English
- Leaves
- 267
- Series
- Methods in Molecular Biology 1346
- Category
- Library
No coin nor oath required. For personal study only.
β¦ Synopsis
This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells.Β
β¦ Table of Contents
Front Matter....Pages i-xi
Back Matter....Pages 1-9
....Pages 11-25
β¦ Subjects
Protein Science
π SIMILAR VOLUMES
<p><p>This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods cap
<span>This detailed volume serves as a collection of methods for single-cell protein analysis, created by combining different protocols, taking advantage of new emerging technologies, and improving upon conventional methods to guide researchers aiming to perform protein analysis in single cells. Ran
<p><p>Today, cells are commonly analyzed <i>en masse</i>, with thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies that in order to learn more about cellular behaviour, it is important to study how individual cells respond, on
<span>This volume explores the latest advancements and techniques used to study cell analysis, their capabilities, and the type of results that can be obtained. The chapters in this book cover topics such as FACS; fluorescence microscopy; organic spectroscopy such as MALDI; inorganic spectroscopy su
<p>The aim of Single Cell Diagnostics: Methods and Protocols is for all readers to extend their knowledge and expertise in analysis of single cells. The book starts with laser assisted cell collection, non-invasive assessment of single cells and moves through the techniques of standard fluorescence