Single Molecule and Single Cell Sequencing

✍ Scribed by Yutaka Suzuki

Publisher: Springer Singapore
Year: 2019
Tongue: English
Leaves: 153
Series: Advances in Experimental Medicine and Biology 1129
Edition: 1st ed.
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This book presents an overview of the recent technologies in single molecule and single cell sequencing. These sequencing technologies are revolutionizing the way of the genomic studies and the understanding of complex biological systems. The PacBio sequencer has enabled extremely long-read sequencing and the MinION sequencer has made the sequencing possible in developing countries. New developments and technologies are constantly emerging, which will further expand sequencing applications. In parallel, single cell sequencing technologies are rapidly becoming a popular platform.

This volume presents not only an updated overview of these technologies, but also of the related developments in bioinformatics. Without powerful bioinformatics software, where rapid progress is taking place, these new technologies will not realize their full potential. All the contributors to this volume have been involved in the development of these technologies and software and have also made significant progress on their applications.

This book is intended to be of interest to a wide audience ranging from genome researchers to basic molecular biologists and clinicians.

✦ Table of Contents

Front Matter ....Pages i-vi
Strategies for Converting RNA to Amplifiable cDNA for Single-Cell RNA Sequencing Methods (Yohei Sasagawa, Tetsutaro Hayashi, Itoshi Nikaido)....Pages 1-17
Integrated Fluidic Circuits for Single-Cell Omics and Multi-omics Applications (Mark Lynch, Naveen Ramalingam)....Pages 19-26
Single-Cell DNA-Seq and RNA-Seq in Cancer Using the C1 System (Masahide Seki, Ayako Suzuki, Sarun Sereewattanawoot, Yutaka Suzuki)....Pages 27-50
Nx1-Seq (Well Based Single-Cell Analysis System) (Shinichi Hashimoto)....Pages 51-61
Quantitation of mRNA Transcripts and Proteins Using the BD Rhapsody™ Single-Cell Analysis System (Eleen Y. Shum, Elisabeth M. Walczak, Christina Chang, H. Christina Fan)....Pages 63-79
An Informative Approach to Single-Cell Sequencing Analysis (Yukie Kashima, Ayako Suzuki, Yutaka Suzuki)....Pages 81-96
Bionano Genome Mapping: High-Throughput, Ultra-Long Molecule Genome Analysis System for Precision Genome Assembly and Haploid-Resolved Structural Variation Discovery (Sven Bocklandt, Alex Hastie, Han Cao)....Pages 97-118
Informatics for PacBio Long Reads (Yuta Suzuki)....Pages 119-129
Challenges of Single-Molecule DNA Sequencing with Solid-State Nanopores (Yusuke Goto, Rena Akahori, Itaru Yanagi)....Pages 131-142
On-Site MinION Sequencing (Lucky R. Runtuwene, Josef S. B. Tuda, Arthur E. Mongan, Yutaka Suzuki)....Pages 143-150

✦ Subjects

Biomedicine; Human Genetics; Bioinformatics

📜 SIMILAR VOLUMES

Single Cell Methods: Sequencing and Prot

📁 Single Cell Methods: Sequencing and Proteomics

✍ Valentina Proserpio 📂 Library 📅 2019 🏛 Springer New York; Humana 🌐 English

This volume provides a comprehensive overview for investigating biology at the level of individual cells. Chapters are organized into eight parts detailing a single-cell lab, single cell DNA-seq, RNA-seq, single cell proteomic and epigenetic, single cell multi-omics, single cell screening, and

Single Cell Sequencing and Systems Immun

📁 Single Cell Sequencing and Systems Immunology

✍ Xiangdong Wang (eds.) 📂 Library 📅 2015 🏛 Springer Netherlands 🌐 English

The volume focuses on the genomics, proteomics, metabolomics, and bioinformatics of a single cell, especially lymphocytes and on understanding the molecular mechanisms of systems immunology. Based on the author’s personal experience, it provides revealing insights into the potential applicatio

Single & single [inglés]

📁 Single & single [inglés]

✍ Le Carré, John ( 📂 Library 📅 2000;2006 🏛 Hodder General Publishing Division;Holdder and Sto 🌐 English

A corporate lawyer from the House of Single & Single is shot dead in cold blood on a Turkish hillside for crimes that he does not understand. A children's entertainer in Devon is hauled to his local bank late at night to explain a monumental influx of cash. A Russian freighter is arrested in the Bla

Single and Ready to Jingle

📁 Single and Ready to Jingle

✍ Piper Rayne 📂 Fiction 🌐 English

Hydrocarbon and Lipid Microbiology Proto

📁 Hydrocarbon and Lipid Microbiology Protocols: Single-Cell and Single-Molecule Methods

✍ Terry J. McGenity, Kenneth N. Timmis, Balbina Nogales (eds.) 📂 Library 📅 2016 🏛 Springer-Verlag Berlin Heidelberg 🌐 English

This Volume presents relevant single-cell and single-molecule approaches in the study of microbes producing and utilizing hydrocarbons and lipids. While generically applicable for all microorganisms, the approaches described are, wherever possible, adapted to the field of study of hydrocarbon and

Single-cell Sequencing and Methylation:

📁 Single-cell Sequencing and Methylation: Methods and Clinical Applications

✍ Buwei Yu, Jiaqiang Zhang, Yiming Zeng, Li Li, Xiangdong Wang 📂 Library 📅 2020 🏛 Springer Singapore;Springer 🌐 English

With the rapid development of biotechnologies, single-cell sequencing has become an important tool for understanding the molecular mechanisms of diseases, defining cellular heterogeneities and characteristics, and identifying intercellular communications and single-cell-based biomarkers. Provi