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Single-cell Gel/Comet Assay Applied to the Analysis of UV Radiation–induced DNA Damage in Rhodomonas sp. (Cryptophyta)

✍ Scribed by Miguel P. Sastre; María Vernet; Scott Steinert

Book ID
111836689
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
108 KB
Volume
74
Category
Article
ISSN
0031-8655

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The alkaline single cell gel electrophoresis (SCGE) or "comet" assay under alkaline conditions was used to measure DNA damage in the liver cells of B6C3F1 male mice exposed to 2,4-dimethylaniline and 2,4,6-trimethylaniline. Cells embedded in agarose were lysed, subjected briefly to an electric field