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Detection of mineral-dust-induced DNA damage in two mammalian cell lines using the alkaline single cell gel/comet assay

✍ Scribed by Bao-zhen Zhong; Wen-zong Whong; Tong-man Ong


Book ID
117767460
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
192 KB
Volume
393
Category
Article
ISSN
1383-5718

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The alkaline single cell gel electrophoresis (SCGE) or "comet" assay under alkaline conditions was used to measure DNA damage in the liver cells of B6C3F1 male mice exposed to 2,4-dimethylaniline and 2,4,6-trimethylaniline. Cells embedded in agarose were lysed, subjected briefly to an electric field