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Detection of DNA lesions induced by chemical mutagens using the single-cell gel electrophoresis (Comet) assay.: 2. Relationship between DNA migration and alkaline condition

✍ Scribed by Youichi Miyamae; Kouichi Iwasaki; Naohide Kinae; Shuji Tsuda; Michiko Murakami; Makiko Tanaka; Yu F Sasaki

Book ID: 117767457
Publisher: Elsevier Science
Year: 1997
Tongue: English
Weight: 65 KB
Volume: 393
Category: Article
ISSN: 1383-5718
DOI: 10.1016/s1383-5718(97)00091-0

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The alkaline single cell gel electrophoresis (SCGE) or "comet" assay under alkaline conditions was used to measure DNA damage in the liver cells of B6C3F1 male mice exposed to 2,4-dimethylaniline and 2,4,6-trimethylaniline. Cells embedded in agarose were lysed, subjected briefly to an electric field