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Detection of chemically induced DNA lesions in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow) using the alkaline single cell gel electrophoresis (Comet) assay

✍ Scribed by Yu F Sasaki; Shuji Tsuda; Fusako Izumiyama; Emi Nishidate

Book ID: 117767388
Publisher: Elsevier Science
Year: 1997
Tongue: English
Weight: 402 KB
Volume: 388
Category: Article
ISSN: 1383-5718
DOI: 10.1016/s1383-5718(96)00133-7

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The alkaline single cell gel electrophoresis (SCGE) or "comet" assay under alkaline conditions was used to measure DNA damage in the liver cells of B6C3F1 male mice exposed to 2,4-dimethylaniline and 2,4,6-trimethylaniline. Cells embedded in agarose were lysed, subjected briefly to an electric field