𝔖 Bobbio Scriptorium
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Single amino acid substitutions affecting the specificity of the fungal ribotoxin mitogillin

✍ Scribed by Richard Kao; Julian Davies


Book ID
117111974
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
502 KB
Volume
466
Category
Article
ISSN
0014-5793

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Single amino acid replacements affecting
✍ Matsumura, Masazumi ;Kataoka, Shiro ;Aiba, Shuichi πŸ“‚ Article πŸ“… 1986 πŸ› Springer 🌐 English βš– 373 KB

Amino acid residues of the carboxyl-terminal region of kanamycin nucleotidyltransferase were modified using segment-directed mutagenesis. Six different mutant enzymes with single amino acid replacements were selected out of 59 clones by DNA sequence analyses. The mutant enzymes were purified and it