𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Simultaneous topography and recognition imaging on endothelial cells

✍ Scribed by L. A. Chtcheglova; P. Hinterdorfer

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
675 KB
Volume
24
Category
Article
ISSN
0952-3499

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Determining the landscape of specific binding sites on biological samples with high spatial accuracy (in the order of several nanometres) is an important task in many fields of biological science. During the past five years, dynamic recognition imaging (e.g. simultaneous topography and recognition (TREC) imaging) has proven to be a powerful technique in biophysical research. This technique becomes an indispensable tool for high‐resolution receptor mapping as it has been successfully demonstrated on different biomolecular model systems. In these studies, the topographical imaging of receptor molecules is combined with molecular recognition by their cognate ligands bound to the atomic force microscope (AFM) tip via a flexible and distensible tether. In this review, we describe the principles of TREC imaging and provide a flavour of its recent application on endothelial cells. Copyright © 2011 John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

AFM functional imaging on vascular endot
AFM functional imaging on vascular endothelial cells
✍ Lilia A. Chtcheglova; Linda Wildling; Jens Waschke; Detlev Drenckhahn; Peter Hin 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 606 KB

## Abstract Vascular endothelial (VE)‐cadherin is predominantly responsible for the mechanical linkage between endothelial cells, where VE‐cadherin molecules are clustered and linked through their cytoplasmic domain to the actin‐based cytoskeleton. Clustering and linkage of VE‐cadherin to actin fil

Simultaneous measurement of endothelial
Simultaneous measurement of endothelial cell damage, elastase release and chemiluminescence response during interaction between polymorphonuclear leukocytes and endothelial cells
✍ Jonas, Eleonore ;Dwenger, Alexander ;Lueken, Britta ;Boehme, Ulrich 📂 Article 📅 1991 🏛 John Wiley and Sons ⚖ 650 KB

Using cultured human umbilical cord vein endothelial cells and human blood neutrophils, the interaction between neutrophils and endothelial cells, in vitro, was studied. The aim of the study was t o examine whether a respiratory burst stimulation by neutrophils would be observed by neutrophil/endoth

Cervical cell recognition and morphometr
Cervical cell recognition and morphometric grading by image analysis
✍ James W. Bacus 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 787 KB

Cervical cell recognition by morphometric image analysis was compared to human visual cell recognition on the same 6,375 cells from 40 dysplastic, CIS, invasive, and 10 normal pap smears. The experimental approach defined receiver operating characteristic (ROC) curves for morphometric image analysis