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Localization of Single Avidin–Biotin Interactions Using Simultaneous Topography and Molecular Recognition Imaging

✍ Scribed by Andreas Ebner; Ferry Kienberger; Gerald Kada; Cordula M. Stroh; Manfred Geretschläger; A. S. M. Kamruzzahan; Linda Wildling; W. Travis Johnson; Brian Ashcroft; Jeremy Nelson; Stuart M. Lindsay; Hermann J. Gruber; Peter Hinterdorfer

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 188 KB
Volume: 6
Category: Article
ISSN: 1439-4235
DOI: 10.1002/cphc.200400545

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Fourier transform infrared reflection-absorption spectroscopy (FT-IRRAS) was successively used to monitor the covalent immobilization of biotin molecules onto a planar gold substrate covered with a self-assembled monolayer of cystamine and to transduce the molecular recognition of avidin and biotin.