Simultaneous measurement of endothelial cell damage, elastase release and chemiluminescence response during interaction between polymorphonuclear leukocytes and endothelial cells

✍ Scribed by Jonas, Eleonore ;Dwenger, Alexander ;Lueken, Britta ;Boehme, Ulrich

• Publisher: John Wiley and Sons
• Year: 1991
• Weight: 650 KB
• Volume: 6
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/bio.1170060106

✦ Synopsis

Using cultured human umbilical cord vein endothelial cells and human blood neutrophils, the interaction between neutrophils and endothelial cells, in vitro, was studied. The aim of the study was t o examine whether a respiratory burst stimulation by neutrophils would be observed by neutrophil/endothelial cell interaction and whether the respiratory burst stimulation of neutrophils by endothelial cells could be enhanced by lipopolysaccharide stimulation o f neutrophils. The second aim was whether such an effect, or secretion of elastase, could cause a n endothelial cell damage in vitro.

Chemiluminescence as an indicator of oxygen-derived metabolites produced by neutrophils, elastase release by neutrophils, and endothelial cell damage, based on "'

In-oxine release from labelled endothelial cells, were measured simultaneously. The present investigation demonstrates that neutrophils can be directly stimulated by endothelial cells. A further amplification of this process following lipopolysaccharide priming up t o 10 ng/ml blood could be demonstrated. A slight endothelial cell damage occurs following neutrophil stimulation, although elastase secretion does not increase during interaction between neutrophils and endothelial cells. These results raise the possibility that oxygen-derived metabolites rather than elastase contribute t o an endothelial cell damage which might occur in conditions such as endotoxin-induced adult respiratory distress syndrome.