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Simultaneous quantitative determination of sucrose, raffinose and stachyose by invertase hydrolysis and gas—liquid chromatography

✍ Scribed by Clive W. Ford

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
314 KB
Volume
30
Category
Article
ISSN
0022-5142

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✦ Synopsis

Abstract

A method for small‐scale (ca 0.5 mg) quantitative determination of sucrose, raffinose and stachyose is described. After invertase hydrolysis, glucose, fructose and melibiose were estimated by gas‐liquid chromatography of the trimethylsilyl derivatives. Sucrose and raffinose were directly estimated from glucose and melibiose levels, respectively. Stachyose values were calculated from the amounts of fructose remaining after deductions were made for the calculated sucrose and raffinose levels. The accuracy of the method from known saccharide mixtures was 6% for sucrose, 3% for raffinose and 10% for stachyose. The method was also shown to be able to simultaneously determine inositols and inositol galactosides.

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