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A new method for qualitative and quantitative determination of di- and polyamines in animal tissues by gas-liquid chromatography

✍ Scribed by Simone Beninati; Claudia Sartori; Maria Paola Argento-Ceru

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 392 KB
Volume: 80
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90629-7

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✦ Synopsis

A gas chromatography method for qualitative and quantitative determination of di-and polyamines from animal tissues was developed. The polyamines were extracted either from tissue homogenates or from standard solutions with the butanol method, opportunely modified in order to improve both the recoveries and the reproducibility of the results. Chromatography was performed on a GLC column prepared with Corning glass beads coated with 1% KOH and 4% Carbowax 20 M, with o-amphetamine and benzoamphetamine as internal standards. This column permits a good separation and a nearly complete recovery of di-and polyamines.

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