Validation of a method for the determination of salbutamol in animal urine by gas chromatography-mass spectrometry and its application to treated lamb samples

✍ Scribed by Carmen Gabiola; María A. García-Calonge; María P. Portillo; J. Alfredo Martínez; Antonio S. del Barrio

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 427 KB
• Volume: 8
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1996)8:5<361::aid-mcs6>3.0.co;2-#

✦ Synopsis

The validation of a fast, reliable, and specific method for the screening, confirmation, and determination of salbutamol in urine with (total) and without (free fraction) a hydrolysis step was developed. Sample urine from different animal species was extracted using Bond Elut certify columns. The described method includes enzymatic hydrolysis, solid-phase extraction, trimethyl silyl derivatives formation, and final determination and identification by gas chromatography-mass spectrometry. Good linearity was obtained between 2 and 40 pg/L. The recovery was 85%, and the limit for identifcation of salbutamol, based on the simultaneous detection of four diagnostic ions, was established at 2 pg/L. Repeatability and within-laboratory reproducibility were 4.4% and 7.1% (percent standard deviation), respectively. Salbutamol was found mainly in the conjugated form in lamb urine (99%). Total salbutamol concentration in lamb urine was compared with those obtained by ELISA.