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Simultaneous gas chromatography of volatile and non-volatile carboxylic acids as tert.-Butyldimethylsilyl derivatives

✍ Scribed by Kyoung R. Kim; Mi K. Hahn; Albert Zlatkis; Evan C. Horning; Brian S. Middleditch

Book ID
104144790
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
811 KB
Volume
468
Category
Article
ISSN
1873-3778

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✦ Synopsis

Solid phase extraction with subsequent ion pair formation and silylation was investigated for the simultaneous trace enrichment of volatile and non-volatile carboxylic acids from complex aqueous samples. The solid phase extraction of acids was performed using Chromosorb P as the solid sorbent. The ether eluate was treated with triethylamine. The resulting triethylammonium salts of the acids were converted to stable tert.-butyldimethylsilyl (TBDMS) derivatives, which were analyzed by gas chromatography (GC) and GC-mass spectrometry. The characteristic M -57 ions in the mass spectra of TBDMS derivatives enabled rapid identification of acids. The application of the method to the organic acid profiling of urine and saliva samples is demonstrated.

πŸ“œ SIMILAR VOLUMES

Analysis of all protein amino acids as t
Analysis of all protein amino acids as their tert.-butyldimethylsilyl derivatives by gas-liquid chromatography
✍ Chin-Joo Goh; Kenneth G. Craven; James R. Lepock; Erwin B. Dumbroff πŸ“‚ Article πŸ“… 1987 πŸ› Elsevier Science 🌐 English βš– 491 KB

A gas chromatographic method for the separation and quantitation of the 20 protein amino acids is described using N-methyl-N(tert.-butyldimethylsilyl)trinuoroacetamide, with 1% tert.butyldimethylchlorosilane as catalyst, to prepare the tert.-butyldimethylsilyl amino acid derivatives. Alkylsilylation