Methylmalonic acid, succinic acid, and other dicarhoxylic acids have been extracted and partially purified from serum and urine using ether extraction and high-performance liquid chromatography. The t-butyldimethylsilyl derivatives were prepared and analyzed using capillary gas chromatography-mass s
✦ LIBER ✦
Quantification of methylmalonic acid in serum measured by capillary gas chromatography—mass spectrometry as tert.-butyldimethylsilyl derivatives
✍ Scribed by Jean Straczek; Franck Felden; Brigitte Dousset; Jean Louis Gueant; Francine Belleville
- Publisher
- Elsevier Science
- Year
- 1993
- Weight
- 369 KB
- Volume
- 620
- Category
- Article
- ISSN
- 0378-4347
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## Abstract A simple, single‐step derivatization technique is presented for capillary GC‐FID and GC‐MS separation and identification of common protein and non‐protein constituents of natural peptides as their __tert__‐butyldimethylsilyl (TBDMS) derivatives. The __tert__‐butyldimethyl‐silylation of
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