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Simultaneous electroelution and concentration of DNA fragments from agarose gels

โœ Scribed by William G. Stroop

Book ID
107714580
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
281 KB
Volume
169
Category
Article
ISSN
0003-2697

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We describe a procedure for the electroelution of nucleic acids from agarose or acrylamide gels. The method employs a combination of low-and high-ionic-strength buffers so that nucleic acids electroelute under a high-voltage gradient but accumulate over a high-salt barrier. By this means electroelut