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Simultaneous Determination of Vanillylmandelic, Homovanillic and 5-Hydroxyindoleacetic Acids in Human Urine by Thin Layer Chromatography

✍ Scribed by G. Alemany; A. Gamundí; C. Rosselló; R. Rial

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 198 KB
Volume: 10
Category: Article
ISSN: 0269-3879
DOI: 10.1002/(sici)1099-0801(199605)10:3<144::aid-bmc570>3.0.co;2-s

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✦ Synopsis

A TLC method for the simultaneous analysis of vanillylmandelic, homovanillic and 5-hydroxyindole-3-acetic acids in urine is described. The sample is cleaned up through a cyano minicolumn and extracted with diethyl ether. The acids are resolved by high-performance TLC, visualized by Folin Ciocalteau reagent and quantificated by densitometry at 600 nm with P(4-hydroxy-3-phenyl) acetic acid as the internal standard.

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