Determination of Petroselinic, cis-Vaccenic and Oleic Acids in Some Seed Oils of the Umbelliferae by Silver Ion Thin Layer Chromatography of their Phenacyl Esters
✍ Scribed by Boryana Nikolova-Damyanova; Svetlana Momchilova; William W. Christie
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 474 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0958-0344
No coin nor oath required. For personal study only.
✦ Synopsis
A method for the separation and determination of ginsenosides Rgl and Rbl in ginseng royal jelly oral liquid has been developed. It uses thin layer chromatography on silica gel plates developed with the lower clear phase of ch1oroform:methanol:water (65:35:10), following macro-reticular resin treatment to remove honey, royal jelly etc. Quantitation was performed by scanning densitometry at 650 nm after post-chromatographic treatment with a 20% solution of phosphomolybdic acid in ethanol. The linearity ranges of the calibration curves of both ginsenosides were 10&1400 ng with regression coefficients greater than 0.99. The detection limits were found to be 20 ng per spot for both compounds. Recoveries were 94.8-106.9%, and the relative standard deviations were 0.68-5.37% for the overall procedure. The method is sensitive and relatively precise, and has been successfully applied in monitoring or controlling the quality of a commercially available dietetic preparation.