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Simultaneous determination of two antioxidants, uric and ascorbic acid, in animal tissue by high-performance liquid chromatography

✍ Scribed by G. Barja de Quiroga; M. López-Torres; R. Pérez-Campo; C. Rojas

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
454 KB
Volume
199
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rapid and simple method for the simultaneous analysis of uric and ascorbic acid in extracts of animal tissue is described. The method uses reversed-phase ion-pair chromatography with ultraviolet detection. The technique allows efficient separation of both acids while showing high selectivity, recovery, reproducibility, and sample stability. Calculated levels of both substances in mouse liver tissue were 1.00 +/- 0.05 mumol ascorbic acid/g and 130 +/- 5 nmol uric acid/g.

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