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Simultaneous determination of myocardial nucleotides, nucleosides, purine bases and creatine phosphate by ion-pair high-performance liquid chromatography

✍ Scribed by Walter Fürst; Seth Hallström


Publisher
Elsevier Science
Year
1992
Weight
391 KB
Volume
578
Category
Article
ISSN
0378-4347

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Nucleotides, nucleosides, and purine bases were extracted from human endomyocardial biopsies, freeze-clamped rat hearts, and porcine coronary sinus plasma. Perchloric acid extracts were neutralized with Freon-trioctylamine and analyzed at 250 nm by reverse-phase ion-pairing high-performance liquid c