Nucleotide preparation from cells and determination of nucleotides by ion-pair high-performance liquid chromatography
β Scribed by Michael Gebelein; Gabriele Merdes; Martin R. Berger
- Publisher
- Elsevier Science
- Year
- 1992
- Weight
- 373 KB
- Volume
- 577
- Category
- Article
- ISSN
- 0378-4347
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Procedures for the complete extraction and isocratic high-performance liquid chromatography analysis of cellular nucleoside di-and triphosphates are reported. Maximum nucleotide recovery from cultures of the ciliate Tetrahymenapyriformis is achieved if (a) cells are cooled to 4Β°C prior to harvest; (