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Determination of nucleotides, nucleosides and nucleobases in cells of different complexity by reversed-phase and ion-pair high-performance liquid chromatography

โœ Scribed by Andreas Werner; Werner Siems; Heike Schmidt; Iris Rapoport; Gerhard Gerber; Ruslan T. Toguzov; Yurii V. Tikhonov; Alexej M. Pimenov

Publisher
Elsevier Science
Year
1987
Weight
559 KB
Volume
421
Category
Article
ISSN
0378-4347

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A sensitive and specific assay for measurement of adenine nucleotides and adenosine by paired-ion high-performance liquid chromatography is described. The I,N6-ethenoderivatives of ATP (c-ATP), ADP (+ADP), AMP (c-AMP). and adenosine (c-Ado), formed by reaction with chloroacetaldehyde at 37ยฐC. were s