Simultaneous derivative spectrophotometric determination of aztreonam and L-arginine in injections

✍ Scribed by Basilio Morelli

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 438 KB
• Volume: 79
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600790317

✦ Synopsis

Mixtures of aztreonam, 2S-[2 alpha,3 beta(Z)]-2-[[[1-(2-amino-4-thiazolyl-2- [(2-methyl-4-oxo-1-sulfo-3-azetidinyl) amino]-2-oxo- ethylidine]amino]oxy]-2-methyl-propanoic acid, and L-arginine are assayed by peak-to-baseline, peak-to-peak, and zero-crossing second-derivative spectrophotometry. Aztreonam is the first of a new class of beta-lactam antibiotics (i.e., the mono-bactam antibiotics, highly effective against aerobic gram-negative bacteria). Beer's law is followed for up to 31 micrograms/mL of aztreonam and L-arginine in the presence of one another. Detection limits at the p = 0.05 level of significance range from 0.11 to 0.37 micrograms/mL. The method was successfully applied to laboratory mixtures and commercial injections containing these substances.