Simultaneous amplification and detection of specific hepatitis B virus and hepatitis C virus genomic sequences in serum samples

✍ Scribed by Sayah Nedjar; Freddie Mitchell; Robin Biswas

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 550 KB
• Volume: 42
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890420221

✦ Synopsis

A sensitive and specific two-stage polymerase chain reaction (PCR) technique was developed for the simultaneous amplification and detection of specific genomic sequences of hepatitis B virus (HBV) and hepatitis C virus (HCV) in serum samples. Initially, HCV-RNA was reverse transcribed to cDNA. This cDNA and DNA from HBV were then co-amplified using primer pairs derived from conserved regions of HBV and HCV nucleotide sequences. The specificity of PCR products was confirmed by liquid hybridization analysis using 32P end-labeled oligomer probes specific for the target HBV and HCV nucleotide sequences. Independent human serum samples, positive and negative by PCR for both HBV-DNA and HCV-RNA, were used as controls. We tested sera from nine donors, of which seven were reactive for HBsAg, anti-HBc, and anti-HCV (multiantigen test), one of whom was reactive for anti-HCV and anti-HBc, and one of whom was reactive for HBsAg and anti-HBc. The assay detected HBV-and HCV-specific genomic sequences in eight of eight sera reactive for both HBV and HCV serological markers and also in the serum that was reactive for HBV markers only.