Simultaneous activity of nucleolus-organizing regions of human and Chinese hamster chromosomes in hybrid somatic cells
β Scribed by G. D. Iashvili; K. N. Grinberg; E. M. Pichugina; V. G. Chernikov
- Publisher
- Springer US
- Year
- 1983
- Tongue
- English
- Weight
- 576 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0007-4888
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About 75 man-Chinese hamster hybrid clones were analysed for their human chromosome complement and simultaneously tested for human enzyme markers. Correlation of the presence of chromosomes and enzyme activity revealed assignments of the PGD linkage group to chromosome 1, ME1, PGM a and IPO-B to 6,
Chromosome material of human and Chinese hamster origin can be stained differentially in human-Chinese hamster translocation chromosomes by use of the Giemsa-11 staining technique.
## Abstract Hybrid clones were obtained between a mouse cell line (3TP) and a temperatureβsensitive Chinese hamster cell line (K12) unable to grow at 40Β° because of a __ts__ defect apparently located at the G1/S transition. The great majority of hybrid clones grew at 40Β°, showing the __ts__ defect
A combination of the silver-staining method of the nucleolus organizer regions (NORs) with a Giemsa-banding method is described. This double staining allows a rapid identification of the NOR-bearing chromosomes.